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抗体消融要领

要领1:我们的抗体(冻干粉)曾经包罗了0.01M PBS、1% BSA和0.1% 防腐剂(叠氮钠或庆大霉素),您只需求到场灭菌的双蒸水就行了。抗体消融后,置于-20℃生存,最好分装后运用,制止重复冻融,可包管最少1年有用;

要领2:也能够只到场一半体积的双蒸水,再用一半体积的甘油补足,置于-20℃生存,如许抗体不会冻,有利于抗体的稳固。


后续实验时,再运用对应的缓冲液稀释成工作液,即用即配。


我们的抗体(冻干粉),正在常温安排下,最少一个月有用;若正在-20℃下生存(推荐),最少三年有用。


Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:

1) Reconstituting by directly adding 100uL of sterile, distilled water.

2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.


附:图解“抗体消融要领”

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